Blotting Principles and Methods



Blotting

•     Blotting is technique in which nucleic acids i.e., RNA and DNA or proteins are transferred onto a specific membrane.

•     It uses hybridization techniques for the identification of the specific nucleic acids and genes.

•     The blotting technique is a tool used in the identification of biomolecules such ad DNA, mRNA and protein during different stages of gene expression.

•     Most of the time, it is executed after electrophoresis.

•     Sometimes, direct blotting is also done.



Types based on Instruments



Wet Blotting




Semi-wet blotting




Dry Blotting






Difference between Southern, Northern and Western blotting